ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2013, Vol. 44 ›› Issue (9): 1454-1461.doi: 10.11843/j.issn.0366-6964.2013.09.017

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Distribution and Relative mRNA Transcription Level of P-gp in Liver, Kidney and Intestines of Piglet

DONG Ling-ling1, GUO Li1, DAI Xiao-hua1,2, SUN Yong1, WANG Li-ping1*   

  1. (1. College of Veterinary Medicine，Nanjing Agricultural University，Nanjing 210095, China; 2. College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052,China)
  • Received:2013-03-28 Online:2013-09-23 Published:2013-09-23

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Abstract:

This study was conducted to investigate the distribution of P-gp and the transcription level of mdr1 mRNA in liver, kidney and intestines of healthy piglets. Five crossbred pigs (Large white × Landrace × Duroc) of approximately 9 weeks of age with an average body weight of 20 kg were used. The localization of P-gp in healthy piglets was studied by immunohistochemisty method. And real-time RT-PCR method was used with gapdh as house-keeping gene to detect the transcription level of mdr1 mRNA. Immunocytochemical method using specific monoclonal and polyclonal antibodies against P-gp confirm high expression in liver, intestines and kidney of the porcine. In the porcine liver, only the apical membrane of the epithelial cells in the bile ducts was positive. In the kidney, immunoreactivity is obvious in the proximal and distal tubules. In the intestines, P-gp was localized in the apical membranes of the enterocytes and the epithelial cells of intestinal gland. The transcription level of mdr1 mRNA ranked from high to low in all tissues was ileum, colon, liver, jejunum, duodenum, rectum, cecum and kidney. The mdr1 mRNA transcription level of ileum was obviously higher than those of cecum and kidney (P=0.005, P=0.001) and significantly higher than those of duodenum and rectum (P=0.017, P=0.014). Also the transcription level of mdr1 mRNA of kidney was significantly lower than those of jejunum, colon and liver respectively (P=0.046, P=0.018, P=0.030). The results indicate that P-gp could be detected in all test tissues，but the mdr1 transcription level is different. The high expression level of P-gp in ileum and liver could play an important role in drug disposition in body and induce drug-drug interaction in clinic.

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